Haven't tried a single 24 volt battery, but something to consider, if using lead acid or AGM they all have 2volt cells, if one dies and you have 2 batteries you replace only one. Weight wise a 24 volt is a lot lighter usually in the mid 40kgs for a 150 amp or 2 x 33kg for 12 volt batteries.
If going LiPo4 then I'd definitely go a single 24 volt as there are all sorts of issues around charging albeit slowly being resolved.
If using lead/AGM have a look at Enerdrives site as there are some very good tips on cabling etc.